Figure 3.

Induction of stress fiber assembly by G2A is inhibited by N19 RhoA. Serum-starved Swiss 3T3 cells were microinjected with pEXV3 G2A and pEXV3 GFP plasmids together with pEXV3 constructs encoding (A) MYC epitope-tagged N19 RhoA, (B) MYC epitope-tagged N17 CDC42, or (C) MYC epitope-tagged N17 Rac1. Cells were fixed 4 h later and stained with 9E10 mAb to detect expression of dominant negative Rho family mutants (Middle) and rhodamine-conjugated phalloidin (Bottom). Large arrows indicate injected cells. (Bar = 50 μm.)