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Reconstitution of functional Gα13 in Gα13 KO MEFs restores their responsiveness to G2A. Gα13 KO MEFs were microinjected with pEXV3 GFP or pEXV3 G2A.GFP together with pCIS Gα13, fixed 4 h later, and stained with rhodamine-conjugated phalloidin (Bottom), and rabbit antiserum against Gα13 to detect ectopically expressed Gα13 (Middle). (Upper) GFP fluorescence. Arrows indicate injected cells. (Bar = 20 μm.)
