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. 2000 Oct 10;97(22):12158–12163. doi: 10.1073/pnas.220420397

Figure 1.

Figure 1

Overexpression of cell wall proteins causes flocculation and adherence to the agar. (A) Ten-milliliter cultures of yeast strains were grown in YPGal overnight, swirled briefly in a Vortex mixer, and photographed immediately and after 30, 60, and 90 min (numbers to the right). Yeast strains: 1, wild type (10560–2B); 2, flo11 (WY168); 3, flo11 GAL1-FIG2 (WY297); 4, GAL1-FLO11 (WY334); 5, flo11 GAL1-FLO10 (WY341); and 6, flo11 GAL1-FLO1 (WY340). (B) The same strains used in A were patched to YPD or YPGal plates. After incubation for 5 days, the plates were washed under a stream of water and rephotographed. The wild-type strain is less invasive on a YPGal plate than on a YPD plate. (C) The same strains used in A were grown in YPD or YPGal liquid medium overnight and swirled briefly in a Vortex mixer, and a fraction of the cells were spotted on an agar (2%) plate. The plate was let dry for 30 min, washed, and rephotographed. The flo11 GAL1-FLO1 strain looks denser on the unwashed plate because the cells are flocculent and stick together in large clumps.