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. 2000 Oct 10;97(22):12158–12163. doi: 10.1073/pnas.220420397

Figure 4.

Figure 4

FLO10 can bypass loss of FLO11 function. (A) FLO10 and FLO11 expression are repressed by SFL1. Northern blots of total RNA (10 μg) prepared from strains WT (10560–2B), sfl1 (WY458), sfl1 flo10 (WY462), and sfl1 flo11 (WY466) were hybridized with FLO10, FLO11, and ACT1 probes, respectively. (B) Derepression of FLO10 leads to haploid invasion. Strains WT (10560–2B), flo10 (WY456), sfl1 (WY458), flo11 (WY460), flo10 sfl1 (WY462), flo10 flo11 (WY464), flo11 sfl1 (WY466), and flo10 flo11 sfl1 (WY468) were patched to a YPD plate. After 5 days of incubation, the plate was washed under a stream of water and rephotographed. (C) Derepression of FLO10 promotes filamentous growth. Strains WT (WY454), flo10/flo10 (WY479), sfl1/sfl1 (WY480), flo11/flo11 (WY481), flo10/flo10 sfl1/sfl1 (WY482), flo10/flo10 flo11/flo11 (WY483), flo11/flo11 sfl1/sfl1 (WY484), and flo10/flo10 flo11/flo11 sfl1/sfl1 (WY485) were streaked on a SLAD plate, grown for 7 days, and photographed before and after washing.