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. 2000 Mar;53(3):238–240. doi: 10.1136/jcp.53.3.238

graphic file with name 99164.f2.jpg

Figure 2 Representative picture of the gels corresponding to microsatellites D3S1286, D18S58, and D13SS171. Lanes 1 were DNA obtained from peripheral blood of the patient that presented with vaginal bleeding. Lanes 2 represented DNA extracted from the atrophic endometrium of the same patient as lanes 1. Lanes 3 corresponded to DNA obtained from the presumed contaminant tissue fragment. Lanes 4 represented DNA isolated from the meningioma that was interpreted as the possible source of contamination. Lanes 5 were DNA from peripheral blood of the patient with the meningioma. No amplification was obtained from DNA of the atrophic endometrium (lane 3) for marker D3S1286, because of the scarcity of the material obtained in the biopsy. The results clearly show that the suspicious fragment (lanes 3) did not match the DNA obtained from peripheral blood (lanes 1) and endometrial tissue (lanes 2) of the patient who underwent the endometrial biopsy.