Figure 1.

Schematic depiction of SBE-TAGS genotyping. (Upper) In SBE, a hybrid primer containing a generic sequence tag followed by a locus-specific sequence is hybridized adjacent to the SNP and extended with fluorescent dideoxynucleotides. (Lower) Multiple SBE reactions are performed in solution; each SBE primer is marked by a different unique sequence tag. The multiplex reaction is analyzed after hybridization to a generic tag array. The array is generated by spotting the reverse complements of the sequence tags onto a glass microscope slide.