Table 2.
Estimated Trp protein ratios compared with trpmRNA ratios calculated from microarray data
| Gene | wt, growth in min vs.
Trp
|
trpR2 vs. wt, growth in
min
|
Brady vs. wt, growth in min
|
wt,
growth in IA vs. min
|
|||
|---|---|---|---|---|---|---|---|
| Protein | mRNA | Protein | mRNA | mRNA | Protein | mRNA | |
| trpE | 10 | 5.0 ± 0.2* | 7.7 | 6.3† | 11.4† | 46 | 81 ± 3‡ |
| trpD | 10 | 5.2 ± 0.7 | 7.7 | 3.7 | 8.0 | 46 | 43 ± 0.5 |
| trpC | 3.7 | 2.2 ± 0.5 | 4.8 | 4.4 | 8.7 | 29 | 30 ± 9 |
| trpB | 3.7 | 2.4 ± 0.2 | 4.8 | 4.1 | 6.9 | 29 | 17 ± 4 |
| trpA | 3.7 | 2.4 ± 0.2 | 4.8 | 3.8 | 9.3 | 29 | 15 ± 2 |
wt, Wild type.
*
mRNA values for cultures grown with tryptophan were corrected for the background levels of nonspecific signal detected with mRNA from strains with the trp operon deleted: ΔtrpEA2. Corrections were 43% for trpE mRNA, 54% trpD mRNA, and 10% for trpC, trpB, and trpA mRNAs.
†
Only one pair of cultures was grown under the conditions indicated.
‡
The averages used are for cultures grown in minimal medium + 15 μg/ml indole acrylate for 30 and 60 min. The corresponding predicted protein values are based on the 6-fold increase in expression observed in strains with the leader terminator deleted.