Table 1.
Strains | BR2495 background
|
SK1
background
|
||||
---|---|---|---|---|---|---|
Crossing over, centimorgans
|
||||||
Sporulation efficiency, % | Spore viability, % | Sporulation efficiency, % | Spore viability, % | MAT/CEN3 | CEN3/HIS4 | |
Wild type + vector | 76.0 | 97.9 | 96.0 | 98.9 | 11.6 | 31.9 |
Wild type + Ndt80-OP | 54.6 | 95.8 | 80.4 | 96.5 | 12.4 | 32.7 |
zip1 + vector | 0 | 65.5 | 60.0 | 5.7 | 3.7 | |
zip1 + Ndt80-OP | 18.0 | 44.8 | 54.0 | 48.8 | 8.8 | 4.3 |
Sporulation efficiencies represent the averages of four independent cultures, with 100 cells counted for each culture. For BR2495 spore viability data, 48 tetrads were dissected from each strain. For spore viability and crossover frequencies in wild-type SK1, 190 tetrads were dissected for each strain. For zip1 SK1 strains, at least 450 tetrads were dissected. OP, overproduced.