Figure 5.

Diabetes promotion by CTLA-4-deficient BDC2.5 T cells. (A) CTLA-4^o/+ mice were crossed three times to BDC2.5/NOD mice and intercrossed to obtain BDC2.5-positive CTLA-4^o/o/NOD animals (selected for H-2g7 homozygosity). These offspring were followed for diabetes. (B) Splenocytes from BDC2.5-positive CTLA-4^o/o/NOD mice and CTLA-4⁺ control littermates were transferred into Cα^o/o/NOD recipients. Pancreata from individual recipients were removed at days 2 or 5 after transfer, and paraffin sections stained with hematoxylin/eosin were examined by histology.