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Cytolytic activity of CTL lines generated from PBMC of uninfected subjects by in vitro immunization. PBMC from MTB-uninfected HLA-A*0201 donors were in vitro immunized to generate CTL lines specific for peptides, ThyA30–38 (a–c) and PstA175–83. (d–f). Cell lines (a) N1–091, (c) M7–091, and (f) M7–029 were generated by the method of Tsai et al. (16). Cell lines (b) M23–091, (d) M22–029, and (e) M23–029 were generated by the method of Plebanski et al. (17).
