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Androgen regulation of PCGEM1. LNCaP cells were cultured in RPMI medium 1640 containing 10% (vol/vol) charcoal-stripped FBS for 4 days and were followed by treatment with synthetic androgen (R1881 for 24 h at 10 nanomolar concentrations). Poly(A)+ RNA from treated and untreated cells was analyzed for PCGEM1 expression by Northern blot hybridization as described for Fig. 4.
