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. 2000 Oct 10;97(22):12233–12238. doi: 10.1073/pnas.210253497

Figure 1.

Figure 1

Escort/inhibitor properties. (A) Depiction of fusion proteins including the ABD of RIN1 (amino acids 1–295) and the catalytic domains of SHP1 (SHP1c: amino acids 250–595) and SHP2 (SHP2c: amino acids 253–593). The sequence of RIN1 that encodes a RAS binding domain (RBD) that is eliminated in the fusion proteins. (B) ABD/SHP1c blocks BCR/ABL-induced transformation. Soft agar colony formation of Rat1 cells infected with virus expressing BCR/ABL (p185) and the indicated constructs was assayed and normalized to results from cells receiving BCR/ABL only (566 colonies = 100%). Data represent mean values and standard deviations from triplicate experiments. (C) Soft agar colony formation of Rat1 cells infected with v-src and indicated constructs. (D) PTP activity measured by using immunoprecipitated material from extracts of 293 cells transfected with the indicated constructs.