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. Author manuscript; available in PMC: 2006 Dec 25.
Published in final edited form as: FEBS Lett. 2006 Oct 12;580(26):6047–6054. doi: 10.1016/j.febslet.2006.09.070

Fig. 6. SK1 (H122Y) activity and cleavage.

Fig. 6

(A) Cleavage assay was performed by incubating recombinant cathepsin B with lysates from Hela cells overexpressing wildtype or mutant SK1. Products were analyzed by Western blotting for SK1. (B) Densitometric quantification of the percentage cleavage of wildtype or H122Y SK1 by cathepsin B. (C) Densitometric quantification of cleavage at the P1 amino acid of the first cleavage site of wildtype or H122Y SK1. Comparisons were made to wildtype, which was set at 100%.