Skip to main content
. 2000 Oct 17;97(22):12250–12255. doi: 10.1073/pnas.200366597

Figure 1.

Figure 1

FACS analysis of the purified memory population. Whole tonsillar lymphocytes were depleted of IgD+ naive B cells and CD3+ T cells by MACS. The depletions were 99% efficient. The resulting population was stained for CD10 and IgD expression, and the memory B cells (IgD−, CD10−) were separated from the germinal center B cells (IgD−, CD10+) by FACS. (A) Unfractionated tonsil cells stained with FITC anti-CD20 (a pan-B cell marker) and PE-coupled anti-IgD. (B) FACS reanalysis of the purified populations with FITC anti-CD10 and PE-coupled anti-IgD (Left) or FITC anti-CD20 and PE-coupled anti-CD10 (Right). Note that the purified population is 95% pure B cells, based on restaining for CD20, and contains only 0.1% IgD+ (naive) and 0.5–0.8% CD10+ (germinal center) B cells.