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Sensitivity controls for the RT-PCR analysis. EBV-positive cells from a cell line were spiked into 5 × 106 EBV-negative tonsillar lymphocytes at the numbers indicated before RNA extraction and cDNA synthesis. Each number of cells was tested independently in triplicate. The cell lines used were IB4 for EBNA2, LMP1, and LMP2a and RAEL for EBNA1(Q-K).
