Figure 4.

RT-PCR analysis of titrated memory cells. Memory cells were isolated, and RT-PCR analysis for EBNA-1(Q-K), EBNA-2, LMP-1, and LMP-2a was performed. The number of cells tested for each dilution is shown. For each dilution tested the number of cells was brought up to 5 × 10⁶ by the addition of EBV-negative tonsillar lymphocytes before RNA extraction and cDNA synthesis. EBV-negative tonsillar lymphocytes (5 × 10⁶) were also used as the negative control. The migration points for the PCR products are indicated by arrows.