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Ribonuclease protection assay showing the expression of GAS6 mRNA by supportive and nonsupportive cell lines. Thirty micrograms of total RNA from each clone was hybridized to a radiolabeled antisense GAS6 probe. The capacity of these lines to support hematopoiesis was described in Dormady et al. (11). Line D5.2-499 is a nonsupportive clone that evolved from the previously supportive D5.2 line.
