Figure 5.

Chelerythrine chloride (CCL) specifically inhibits the ability of WT S. typhimurium and purified SipA-HA to induce PMN transepithelial migration. WT S. typhimurium, purified SipA-HA (20 μg/ml), or fMLP (10 nM) were incubated with T84 cells to induce PMN migration (empty bars). The effect of CCL (filled bars) was assessed by incubating T84 cell monolayers in the presence of 5 μM CCL for 30 min at 37°C. Inducers were added to the apical surface of T84 cell monolayers in the continued presence of the drug, and after 1 h (at 37°C), the monolayers were processed for the PMN transmigration assay. Data represent the mean ± SD of triplicate samples: (*, P < 0.05).