GFRα2 −/− mice require more stimulations to reach higher seizure
grades, and the persistence of hyperexcitability is less pronounced.
(A) The number of stimulations to reach different
seizure grades. (B and C) Mean seizure
grade and AD duration, respectively, in response to the first and last
five stimulations during kindling, and after the five test
stimulations. The number of stimulations to exhibit forelimb clonus
(grade 3), generalized seizures (grades 4 and 5), and the fully kindled
state (third grade 5) was significantly increased in GFRα2 −/−
mice (A). The mean seizure grade (B) and
AD duration (C) induced by test stimulations were
significantly lower and shorter, respectively, in GFRα2 −/− mice.
Mean ± SEM. *, P < 0.05, Student's
unpaired t test (A) and one-way ANOVA
followed by Bonferroni/Dunn post hoc test (B and
C) (n = 10 for GFRα2 +/+ and
n = 7 for −/− mice).