Down-regulation of NPY immunoreactivity in hilar interneurons
after test stimulations in kindled animals is not observed in GFRα2
−/− mice. Color photomicrographs showing NPY immunoreactivity in
the dentate hilus of non-kindled (A and
B) and kindled animals (C and
D). (E) The number of NPY-positive
neurons; (F) the optical density of NPY immunoreactivity
in the hilus of nonstimulated or kindled GFRα2 +/+ and −/−
mice. Note the decrease of NPY-positive neurons in the hilus of
kindled, wild-type animals (C), with no alterations in
the knock-out mice (D), as compared with corresponding
controls (A and B, respectively).
Mean ± SEM. *, P < 0.05, Student's
unpaired t test (n = 10 for GFRα2
+/+ and n = 7 for −/− mice). Bar = 160
μm.