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. 2000 Oct 17;97(22):12334–12338. doi: 10.1073/pnas.210384297

Figure 3.

Figure 3

Effects of N-terminal mutations of threonine residues on PKC sensitivity of the Cav1.2 channel. (A) Peak Ba2+ currents recorded every 30 s. PMA was applied during the time indicated by the horizontal black bar. (Inset) Ba2+ currents elicited by a depolarizing step from −80 mV to +10 mV. Current traces such as those in the Inset were taken at time points a and b before and during exposure to PMA. (B) Mean changes in peak current amplitude in response to application of 100 nM PMA for wild-type Cav1.2 (WT) and several N-terminal mutants. The mean changes in current amplitude in response to PMA were as follows: Cav1.2 wild-type, −61.5 ± 7.1%, n = 6; N-terminal deletion [N-del (2–46)], −2.9 ± 1.8%, n = 3; TTAA, −1.5 ± 1.5%, n = 5; T27A, +3.2 ± 4.7%, n = 5; T31A, +0.2 ± 3.5%, n = 3.