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. 2000 Oct 10;97(22):12350–12355. doi: 10.1073/pnas.210276297

Figure 1.

Figure 1

Complementation of UFA auxotrophy of E. coli MH13/pAnFd cells by expression of plant 14:0- and 16:0-ACP DES. E. coli MH13/pAnFd cells were transformed with the expression vector pLac3d (pLac) alone or with pLac3d containing the coding sequences of the milkweed Δ9-16:0-ACP DES (Δ9-16:0), the black-eyed Susan Δ6-16:0-ACP DES (Δ6-16:0), the geranium Δ9-14:0-ACP DES (Δ9-14:0), the castor Δ9-18:0-ACP DES (Castor Δ9-18:0), or the black-eyed Susan Δ9-18:0-ACP DES (BS Δ9-18:0). Transformed cells were grown at 30°C for 40 h either under permissive conditions on media containing oleic acid (+UFA) or under selective conditions on media lacking oleic acid (−UFA) as described in Materials and Methods.