Figure 2.
UFA content of E. coli MH13/pAnFd cells expressing the geranium Δ9-14:0-ACP desaturase (Δ9-14:0), the black-eyed Susan Δ6-16:0-ACP desaturase (Δ6-16:0), or the milkweed Δ9-16:0-ACP desaturase (Δ9-16:0). Cultures were initiated from single colonies and grown at 30°C to OD600 ≈ 0.3–0.5 with IPTG induction and antibiotic selection as described in Materials and Methods. Double bond positions were determined by GC-MS of dimethyl disulfide derivatives of fatty acid methyl esters (21). Minor unsaturated fatty acid components are indicated by a, b, and c: a includes ≤3.5 mol% of 14:1Δ9, 16:1Δ9, and 18:1Δ13; b includes ≤5 mol% of 18:1Δ8; and c includes ≤2 mol% 18:1Δ9 and 18:1Δ13.