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. 2000 Oct 17;97(22):12356–12360. doi: 10.1073/pnas.210214197

Figure 2.

Figure 2

Summary of point mutant phenotypes. A diagram of the IRT1 amino acid sequence is shown with predicted transmembrane domains in the center, numbered I–VIII, predicted cytoplasmic loops above, and predicted extracellular regions below. Residues shaded in pink (Glu-103) are those whose replacement with alanine leads to variants that transport Fe, Mn, and Cd but not Zn; those in blue (Asp-100) are residues whose replacement with alanine leads to variants that transport Zn and Cd but not Fe and Mn, and those in green (Asp-136) transport Zn and reduced levels of Cd. Residues whose replacement with alanine leads to variants with no transport activity are shown in gray (His-96, His-197, Ser-198, His-224, and Glu-228), and those with wild-type transport function are shown in gold (Cys-109, Tyr-295, Asp-300, and Glu-305).