Skip to main content
Infection and Immunity logoLink to Infection and Immunity
. 1996 Mar;64(3):763–768. doi: 10.1128/iai.64.3.763-768.1996

Immunoglobulin A response in murine schistosomiasis: stimulatory role of egg antigens.

O Poulain-Godefroy 1, S Gaubert 1, S Lafitte 1, A Capron 1, J M Grzych 1
PMCID: PMC173835  PMID: 8641779

Abstract

The immunoglobulin G (IgG) and IgA antibody responses to different Schistosoma mansoni antigens have been determined in chronically infected mice as well as in unisexually infected animals. With a panel of enzyme-linked immunosorbent assays (ELISAs), soluble antigens from furcocercariae, adult worms, and eggs were probed with sera collected at 3-week intervals. Bisexually infected animals developed significant IgG and IgA antibody responses to the antigens tested, which increased after egg deposition. In unisexual infections no significant differences were recorded in the IgG antibody profile for furocercaria and adult worm antigens, whereas the IgA antibody response was impaired. Both the IgA and IgG antibody responses toward egg antigens were reduced compared with those in a bisexual infection. Furthermore, a specific mucosal IgA antibody response was observed only in the bisexually infected animals. Histological analysis performed on bisexually infected mice led to the observation of eggs and granulomatous lesions within the Peyer's patch follicles, which are essential sites for the induction of mucosal immunity in the intestine. These data suggest a relationship between egg deposition and the induction of the IgA antibody response toward schistosomes.

Full Text

The Full Text of this article is available as a PDF (624.1 KB).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Balloul J. M., Grzych J. M., Pierce R. J., Capron A. A purified 28,000 dalton protein from Schistosoma mansoni adult worms protects rats and mice against experimental schistosomiasis. J Immunol. 1987 May 15;138(10):3448–3453. [PubMed] [Google Scholar]
  2. Befus D., Lee T., Ernst P., Egwang T., McElroy P., Gauldie J., Bienenstock J. Unique characteristics of local responses in host resistance to mucosal parasitic infections. Vet Parasitol. 1986 Mar;20(1-3):175–194. doi: 10.1016/0304-4017(86)90099-3. [DOI] [PubMed] [Google Scholar]
  3. Buijs J., Lokhorst W. H., Robinson J., Nijkamp F. P. Toxocara canis-induced murine pulmonary inflammation: analysis of cells and proteins in lung tissue and bronchoalveolar lavage fluid. Parasite Immunol. 1994 Jan;16(1):1–9. doi: 10.1111/j.1365-3024.1994.tb00297.x. [DOI] [PubMed] [Google Scholar]
  4. Crabtree J. E., Pullar C. E., Trejdosiewicz L. K., Wilson R. A. Murine intestinal humoral responses in chronic Schistosoma mansoni infections. Scand J Immunol. 1992 Mar;35(3):361–367. doi: 10.1111/j.1365-3083.1992.tb02869.x. [DOI] [PubMed] [Google Scholar]
  5. Davis P. J., Porter P. A mechanism for secretory IgA-mediated inhibition of the cell penetration and intracellular development of Eimeria tenella. Immunology. 1979 Mar;36(3):471–477. [PMC free article] [PubMed] [Google Scholar]
  6. Grezel D., Capron M., Grzych J. M., Fontaine J., Lecocq J. P., Capron A. Protective immunity induced in rat schistosomiasis by a single dose of the Sm28GST recombinant antigen: effector mechanisms involving IgE and IgA antibodies. Eur J Immunol. 1993 Feb;23(2):454–460. doi: 10.1002/eji.1830230223. [DOI] [PubMed] [Google Scholar]
  7. Grzych J. M., Grezel D., Xu C. B., Neyrinck J. L., Capron M., Ouma J. H., Butterworth A. E., Capron A. IgA antibodies to a protective antigen in human Schistosomiasis mansoni. J Immunol. 1993 Jan 15;150(2):527–535. [PubMed] [Google Scholar]
  8. Grzych J. M., Pearce E., Cheever A., Caulada Z. A., Caspar P., Heiny S., Lewis F., Sher A. Egg deposition is the major stimulus for the production of Th2 cytokines in murine schistosomiasis mansoni. J Immunol. 1991 Feb 15;146(4):1322–1327. [PubMed] [Google Scholar]
  9. Kraehenbuhl J. P., Neutra M. R. Molecular and cellular basis of immune protection of mucosal surfaces. Physiol Rev. 1992 Oct;72(4):853–879. doi: 10.1152/physrev.1992.72.4.853. [DOI] [PubMed] [Google Scholar]
  10. Kullberg M. C., Pearce E. J., Hieny S. E., Sher A., Berzofsky J. A. Infection with Schistosoma mansoni alters Th1/Th2 cytokine responses to a non-parasite antigen. J Immunol. 1992 May 15;148(10):3264–3270. [PubMed] [Google Scholar]
  11. Leid R. W., Williams J. F. The immunological response of the rat to infection with Taenia taeniaeformis. I. Immunoglobulin classes involved in passive transfer of resistance. Immunology. 1974 Aug;27(2):195–208. [PMC free article] [PubMed] [Google Scholar]
  12. Lloyd S., Soulsby E. J. The role of IgA immunoglobulins in the passive transfer of protection to Taenia taeniaeformis in the mouse. Immunology. 1978 May;34(5):939–945. [PMC free article] [PubMed] [Google Scholar]
  13. McGhee J. R., Mestecky J., Dertzbaugh M. T., Eldridge J. H., Hirasawa M., Kiyono H. The mucosal immune system: from fundamental concepts to vaccine development. Vaccine. 1992;10(2):75–88. doi: 10.1016/0264-410x(92)90021-b. [DOI] [PubMed] [Google Scholar]
  14. Mosmann T. R., Cherwinski H., Bond M. W., Giedlin M. A., Coffman R. L. Two types of murine helper T cell clone. I. Definition according to profiles of lymphokine activities and secreted proteins. J Immunol. 1986 Apr 1;136(7):2348–2357. [PubMed] [Google Scholar]
  15. Mosmann T. R., Coffman R. L. Heterogeneity of cytokine secretion patterns and functions of helper T cells. Adv Immunol. 1989;46:111–147. doi: 10.1016/s0065-2776(08)60652-5. [DOI] [PubMed] [Google Scholar]
  16. Nedrud J. G., Liang X. P., Hague N., Lamm M. E. Combined oral/nasal immunization protects mice from Sendai virus infection. J Immunol. 1987 Nov 15;139(10):3484–3492. [PubMed] [Google Scholar]
  17. Owen R. L., Ermak T. H. Structural specializations for antigen uptake and processing in the digestive tract. Springer Semin Immunopathol. 1990;12(2-3):139–152. doi: 10.1007/BF00197502. [DOI] [PubMed] [Google Scholar]
  18. Pearce E. J., Caspar P., Grzych J. M., Lewis F. A., Sher A. Downregulation of Th1 cytokine production accompanies induction of Th2 responses by a parasitic helminth, Schistosoma mansoni. J Exp Med. 1991 Jan 1;173(1):159–166. doi: 10.1084/jem.173.1.159. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Ramaswamy K., Befus D. Pulmonary immune responses to Nippostrongylus brasiliensis: isotype-specific antibodies in bronchoalveolar lavage fluids of rats. Parasite Immunol. 1993 Oct;15(10):573–582. doi: 10.1111/pim.1993.15.10.573. [DOI] [PubMed] [Google Scholar]
  20. Smithers S. R., Terry R. J. The infection of laboratory hosts with cercariae of Schistosoma mansoni and the recovery of the adult worms. Parasitology. 1965 Nov;55(4):695–700. doi: 10.1017/s0031182000086248. [DOI] [PubMed] [Google Scholar]
  21. Tomasi T. B., Grey H. M. Structure and function of immunoglobulin A. Prog Allergy. 1972;16:81–213. [PubMed] [Google Scholar]
  22. Underdown B. J., Schiff J. M. Immunoglobulin A: strategic defense initiative at the mucosal surface. Annu Rev Immunol. 1986;4:389–417. doi: 10.1146/annurev.iy.04.040186.002133. [DOI] [PubMed] [Google Scholar]
  23. Velupillai P., Harn D. A. Oligosaccharide-specific induction of interleukin 10 production by B220+ cells from schistosome-infected mice: a mechanism for regulation of CD4+ T-cell subsets. Proc Natl Acad Sci U S A. 1994 Jan 4;91(1):18–22. doi: 10.1073/pnas.91.1.18. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Infection and Immunity are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES