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Desulfonation of SafeBis-DNA in PCR buffers
| Δ Cta | ||||
|---|---|---|---|---|
| PCR | Desulfonation time (min) | FastStart LightCycler mix | QuantiTect kit | qPCR core kit |
| GSTp1-HM | 10 | 2.7 | Nd | Nd |
| 15 | 1.9 | Nd | Nd | |
| 20 | 0.6 | Nd | Nd | |
| 25 | 0.2 | Nd | Nd | |
| 30 | 0.2 | Nd | Nd | |
| TMEFF2-HM | 30 | 0.5 | Nd | Nd |
| Reference-PCR | 30 | 0.4 | 0.4 | 5.2 |
adetermined as (Ct of 1 ng SafeBis DNA)—(Ct of 1 ng Bisulfite DNA).
