Table 2.
Determination of the detection limits of three real-time PCRs on SafeBis and Bisulfite template DNA
| PCR | SafeBis DNA | Bisulfite DNA | ||
|---|---|---|---|---|
| LOD50 (pg)a | 95% CI (pg)b | LOD50 (pg)a | 95% CI (pg)b | |
| GSTP1-HM | 4.9 | 2.3–6.9 | 4.8 | 0.5–8.1 |
| TMEFF2-HM | 7.0 | 3.7–9.3 | 4.1 | 0.0–6.8 |
| Reference-PCR | 5.7 | 1.3–8.3 | 6.9 | 2.7–9.9 |
a50% detection limits were calculated by a Probit regression analysis based on the results using 50, 25, 12, 6 and 0 pg methylated DNA analysed in 16 replicates each.
b95% confidence interval of LOD50 value.