Table 1.
Viral hemorrhagic fever primer panel
| Virus | Reference strain | Gene | Direction | Sequence (5′–3′) | Tm (°C) | Amplicon length (nt) |
|---|---|---|---|---|---|---|
| Zaire Ebola Virus (ZEBOV) | NC_002549 | L Polymerase | F | TTCCCTCCGTTGCCAATGATTAAAGAAC | 59.5 | 149 |
| R | ACTGCATCCCAGCATGTCCTTTC | 59.0 | ||||
| Crimean Congo hemorrhagic fever (CCHV) | U88412 | Nucleocapsid | F | ACTYGTTGCAACWGGCCTTGC | 59.4 | 142 |
| R | CATGYTGTCRGCACTTGCTTTRTCAAC | 60.2 | ||||
| Seoul virus (SEOV) | NC_005236 | Nucleocapsid | F | YGATGARYTGAAGCGCCARCTTGC | 61.7 | 72 |
| R | GTAGGATCCCGRTCYTGCCC | 57.8 | ||||
| Rift Valley fever virus (RVFV) | NC_002045 | NSs | F | ACYGAGGCYATCCTMAGAGGGATTGAC | 61.6 | 66 |
| R | AWYCTCATACATGASRTCAAAGCCTGGCAAC | 62.2 | ||||
| Kyasanur Forest disease virus (KFDV) | NC_004355 | NS5 | F | CGTRTGGARGCCTGGCTGAAAG | 59.6 | 61 |
| R | CRCTGACCAGCATRCGGGT | 58.9 |