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. 2006 Nov 28;34(22):6612–6620. doi: 10.1093/nar/gkl984

Figure 4.

Figure 4

The Zinc-knuckle and RRM domains add significant potency to 9G8 splicing activation. (A) Schematic representation of MS2–9G8 recombinant fusion proteins. MS–9G8 is full-length 9G8 fused in-frame to the bacteriophage MS2 coat protein. Likewise, MS2–ZnRS(9G8) contains only the Zinc-knuckle and RS domain of 9G8, and MS2–RS(9G8) contains only the RS domain of 9G8, fused in-frame to the MS2 protein. (B) Schematic representation of the dsx mini-gene RNA substrate used (dsx200-MS2) with one synthetic MS2-hairpin enhancer element positioned ∼200 nt downstream of the female-specific 3′ splice site. (C) In vitro splicing assays were performed with a dsx mini-gene RNA substrate containing a single MS2-hairpin ∼200 nt from the 3′ splice site (dsx200-MS2) in the presence of recombinant MS2-fusion proteins. Diagrams to the right indicate unspliced, spliced or intermediate products. Average splicing rates for each MS2-fusion protein are listed below the corresponding gel (data from Table 2).