Figure 5.
The RS domains of Tra and Tra2 are essential for splicing activation. In vitro splicing assays were performed with the dsx300-(MS2)2 RNA substrate in the presence of the recombinant MS2-fusion proteins represented by schematic diagrams. (A) Splicing activities for full-length Tra2 (MS2–Tra2) indicated by filled circles, RS1-deletion mutant (MS2-Tra2ΔRS1) indicated by open diamonds and RS2-deletion mutant (MS2-Tra2ΔRS2) indicated by open circles are plotted as a function of time. (B) Splicing activities for full-length Tra (MS2–Tra) indicated by filled circles, RS-deletion mutant (MS2-TraΔRS) indicated by open diamonds and proline-rich region-deletion mutant (MS2-TraΔPro) indicated by open circles are plotted as a function of time. Average splicing rates for these recombinant proteins are listed in Table 2.