Table 1.

Splicing rates promoted by recombinant Tra and Tra2

	RNA substrate
	dsx300-1RE	dsx300-2RE	dsx300-4RE	dsx300-5RE
Tra + Tra2a	0.11	0.31	0.33	0.39
MS2–Tra + Tra2	nd	nd	nd	0.38
Tra + MS2–Tra2	nd	nd	nd	0.19

All rate constants are expressed in units of h⁻¹. Each value is the average from at least three independent splicing reactions consisting of 7–8 time points over a 4 h time period. To derive rate constants, the data at time points following the lag were fit to a first-order rate equation (43). Experimental error for the rate constants is within 50%. nd, not determined.

^aAverage splicing rate per dsxRE with Tra + Tra2 = 0.11 ± 0.05 h⁻¹.