Fig. 6.

Characterization of −/−TXF glial cells. Photomicrographs of immunostained cells. +/+ Schwann cells (SC) and fibroblasts (Fib) were used as positive and negative controls. A, D,G, and J represent −/−TXF Schwann cells, B, E, H, andK are +/+ Schwann cells, and C,F, I, and L are −/− fibroblasts [with the same pattern of expression as +/+ fibroblasts (data not shown)]. A–C show anti-p75NGFR,D–F show anti-S100, G–I show anti-GFAP, and J–L show anti-SMA immunostaining. The −/−TXF Schwann cells are p75NGFR, S100, and GFAP positive. +/+ Schwann cells are also positive for p75NGFR and S100 but show much weaker staining for GFAP (visible in only the boxed inset inH; a longer exposure than H, surroundinginset). Exposure times for G–I were matched. Fibroblasts are strongly positive for SMA. Scale bar, 50 μm.