Fig. 3.
75Se incorporation into the E. coli selenoprotein FDHH. Cells were grown in the presence of [75Se]selenite in TGYEP medium (see Materials and Methods) supplemented with 0.05 mM IPTG anaerobically at 37°C for 24 h. Cell extracts were separated by 10% SDS/PAGE, and the formation of 75Se-containing FDHH was followed by autoradiography. The E. coli strain JS1 was complemented with E. coli SelA (lane 1), empty plasmids (lane 2), Methanocaldococcus jannaschii SepSecS (lane 3), Methanocaldococcus jannaschii SepSecS with PSTK (lane 4), and Methanocaldococcus jannaschii SepSecS, PSTK with Methanococcus maripaludis tRNASec (lane 5).