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. 2006 Dec 5;103(50):18963–18968. doi: 10.1073/pnas.0606146103

Fig. 1.

Fig. 1.

Genome organization of TMV-TMOF containing a read-through sequence. TMV-U1 ORF representing the replicase of 126 and 183 kDa (126K/183K), the cell-to-cell movement protein 30 kDa (30K), and the viral coat protein (CP) was fused to a read-through sequence (5′-TAGCAATTA-3′), a trypsin cleavage site (IEGR), and an A. aegypti TMOF (YDPAPPPPPP) sequence behind a T7 promoter at the 5′ end of the construct. The TMV-TMOF RNA was transcribed by using T7 RNA polymerase, and the transcribed RNA was mechanically inoculated onto Ni. tabacum (L) cv. xanthu. Gene distances are not proportional to gene sizes.