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. 2006 Dec 1;103(50):18975–18980. doi: 10.1073/pnas.0608565103

Fig. 4.

Fig. 4.

Histological analyses and AR staining in testis of AR+/y, G-AR−/y, and T-AR−/y mice. Histology of testes by H&E staining (A–C) and immunostaining (D–F) of AR protein in testicular sections from 14-week-old AR+/y, G-AR−/y, and T-AR−/y mice. Four to six 14-week-old mice from individual groups were killed, and testes were excised for histology section. Three of the G-AR−/y mice have been confirmed with the 100% AR knockout in germ cells by genotyping of female offspring (Table 1). I–XII in each image in A and B indicate the specific stage of spermatogenesis. There is no difference in testicular morphology between AR+/y and G-AR−/y mice. In contrast, testes from T-AR−/y mice (C) showed maturation arrest at pachytene spermatocytes (arrowheads) and apoptotic-like bodies (arrows) in some seminiferous tubules and Sertoli cells only (with degeneration, x) in the other tubules. (Scale bars: A and B, 30 μm; C, 15 μm.) In testes from both AR+/y (D) and G-AR−/y (E) mice, the AR protein was located in the nuclei of Sertoli cells (black arrows), Leydig cells (arrowheads), and peritubular myoid cells (blue arrows). The testis from the T-AR−/y mouse (F) was used as a negative control to confirm the specificity of AR staining. (Scale bar: 30 μm.)