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. 2006 Dec 5;103(50):19087–19092. doi: 10.1073/pnas.0602715103

Fig. 5.

Fig. 5.

Effect of AT1R on eNOS expression and activity. (A) Expression of eNOS protein in BAECs transfected with either WT or the N111G mutant AT1R expression plasmid after 1 μM Ang II or SII-Ang II treatment for 24 h. Control is recombinant eNOS purified from insect cells. (B) Effect of 1 μM ligand stimulation of AT1R for 45 min on eNOS activity. Enzymatic activity of eNOS in WT and N111G mutant AT1R-transfected BAEC lysates (n = 3) is shown. Bars show mean ± SEM. (C) AT1R specificity of eNOS protein expression in BAEC is demonstrated by treatment with L-158809; UT indicates untransfected BAEC.