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. 2006 Dec 4;103(50):19140–19145. doi: 10.1073/pnas.0603788103

Fig. 1.

Fig. 1.

Truncating the acidic domain of CHMP3 triggers anti-HIV-1 budding activity. (A) Schematic representation of FLAG-tagged CHMP3 constructs and their effects on HIV-1 particle production. 293T cells were transfected with full-length HIV-1 proviral DNA (1.5 μg) and vectors expressing full-length or truncated CHMP3-FLAG (500 ng). Virion production and Gag expression levels were compared by Western blotting with anti-CA serum, and CHMP3 expression levels were compared by Western blotting with anti-FLAG. (B) CHMP31–200FLAG inhibits HIV-1 particle production less potently than CHMP31–150FLAG when expressed at a comparable level. 293T cells were transfected with proviral DNA (1.5 μg) and expression vectors for CHMP31–200FLAG (100, 50, 25, or 12.5 ng) or CHMP31–150FLAG (500 ng).