Fig. 1.

The sequence between −4000 and −3018 of SLC6A2 promoter contains the noradrenergic cell-specific transcriptional element. The fragment between −4000 and −3018 was subcloned in either sense or antisense orientation 5′ of the pBLCAT2 or 5′ of the pNET133(i)CAT (20). The reporter constructs were transfected into the NET-positive SK-N-BE (2)C cells and NET-negative HeLa cells, and the normalized CAT activity of each construct was expressed relative to the vector pBLCAT2 (A) and pNET133(i)CAT (B), respectively.