The sequence of the zebrafish dlx2b 4-kb 5′ region contains a phylogenetically conserved domain and several potential transcription factor binding sites. (A) Diagram of the zebrafish dlx2b:GFP construct. Pea3 (Fgf), Smad (Bmp), and Dlx consensus binding sites are indicated. The region conserved with tetrapods is highlighted with a black box. (B) Alignment of the conserved 61-bp region between mouse (Mm), frog (Xenopus tropicalis, Xt), and zebrafish (Dr) sequences; ∗ indicates sequence identity. Potentially conserved transcription factor binding sites are underlined and labeled.