Fig. 3.

Alteration of PIP₂ metabolism in presenilin FAD mutant cells. (A) Lipid kinase and TLC analysis of extracts prepared from HEK293 cells stably transduced with either WT or FAD mutant (ΔE9, L286V) PS1. PIP, phosphatidylinositol 4-monophosphate. (B) Quantification of TLC data on PIP₂. Data are from five independent experiments, and they are expressed as mean ± SEM. (C) Quantification of TLC analyses on extracts prepared from HEK293 cells stably expressing WT or FAD mutant (N141I) PS2. Data are from at least three independent experiments, and they are expressed as mean ± SEM. ∗, P < 0.01; ∗∗, P < 0.05.