Fig. 1.
Decreasing SOD1 in muscle does not improve grip strength in SOD1G93A mice. (A and B) Genes encoded in AAV are expressed in muscle and in motor neurons after retrograde transport. Genes encoded in lentivirus are expressed in muscle only. One hindlimb of 40-day-old SOD1G93A mice was injected with either lentivirus or AAV containing a siRNA directed against SOD1. The siRNA construct was identical in each case. (C) Protein blots of SOD1 and GFP levels in gastrocnemius from lentivirus- and AAV-injected SOD1G93A mice (three representative animals in each group). (D) Grip strength of the right treated hindlimb in AAV siRNA- vs. lentivirus siRNA-treated animals. (E) Wet weights of 98-day-old SOD1G93A mice treated with AAV siRNA vs. lentivirus siRNA (n = 10, average ± SE).