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. 2006 Dec 12;103(51):19546–19551. doi: 10.1073/pnas.0609411103

Fig. 3.

Fig. 3.

Follistatin promotes myoblast proliferation in vitro. In vitro testing was performed to confirm the biological activity of follistatin to inhibit myostatin. (A) Schematic of follistatin in AAV vector and plasmids used for AAV production. CMV represents the cytomegalovirus promoter with a splice donor/acceptor sequence. Poly(A) represents the poly-adenylation sequence. The vector is flanked by the two AAV inverted terminal repeats from serotype 2. Rep2Cap1 represents the plasmid used for packaging the AAV into capsid serotype 1 along with the adenoviral helper plasmid. (B) Proliferation assay of myoblasts (C2C12) cultured in growth media in the presence or absence of myostatin (3 μg/ml) mixed with conditioned media from AAV-transduced cells expressing either RFP or follistatin (FS) (n = 4 average ± SE).