Fig. 5.

Both CheB42a and llz transcripts are expressed preferentially in male-specific sensory structures. (A) Real-time quantitative RT-PCR. Transcripts of both CheB42a and llz were enriched in male appendages relative to other body parts or females by ANOVA (P < 0.001; n = 6 per group). Data are mean ± SEM. (B) Northern blot analysis of total RNA from adult tissues. The same membrane was probed for both genes with stripping between hybridizations. The blot of llz required much longer exposure of the membrane, suggesting lower-level expression than CheB42a. Data for male appendages are the same as those shown in Fig. 2A. (C) Genetic elimination of cells by expressing the cell death-inducer rpr under the CheB42a/llz promoter reduced abundance of both CheB42a and llz transcripts in male appendages (ANOVA P < 0.02; n = 4 per group). (D–G) In situ hybridization for CheB42a (D) and llz (E) in embryos. Individual embryos were hybridized simultaneously with CheB42a and llz antisense probes labeled with two different fluorophores or with the corresponding sense probes. The front of embryo is in upper right. Arrowheads indicate location of signal, likely in neurons. (F and G) CheB42a and llz sense controls. Arrows in F and G indicate nonspecific fluorescence in trachea. (H) The CheB42a-llz locus is expressed in third-instar larvae in a single lateral multidendritic neuron (md) plus two external sensory neurons (es) that project to the chemosensitive terminal organ (TO). Dorsal organ (DO), which shows autofluorescence, also is indicated. Larva is outlined by dotted line.