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. 2006 Dec 15;103(52):19788–19793. doi: 10.1073/pnas.0609385103

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© 2006 by The National Academy of Sciences of the USA

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Fig. 3. — Localization of SAHH with F-actin in pseudopods of motile Dictyostelium. (A) Localization of F-actin (rhodamine-phalloidin, red) and endogenous SAHH (anti-SAHH, green) in vegetative (Top), polarized vegetative (Middle), and developed chemotaxing (Bottom) cells. Vegetative cells are in nutrient, HL5 medium; polarized vegetative cells are motile cells in nutrient medium; and developed chemotaxing cells are cells that were pulsed for up to 8 h with cAMP in nonnutrient buffer. (B) GFP-SAHH is localized at the front of live, chemotaxing cells. White dots show the position of the tip of the micropipette filled with 1 μM cAMP. (C) GFP-SAHH becomes diffuse when F-actin in polarized cells is depolymerized by 2 μM latrunculin A. (D) Both GFP-coronin and GFP-SAHH localize with F-actin in pseudopods, but only coronin is in filopodia and immediately under the plasma membrane.