Fig. 5.

Electron micrographs showing morphogenesis during cell division. (A) Septal formation in untreated B. subtilis. (B) Multiple septation 30 min after treatment with 5 μg/ml nisin. (C–F) Aberrant morphogenesis after treatment with 50 μg/ml nisin. (G) The formation of minicells. (C–F) Multiple septation with abnormal septal orientation and cell swelling (C), helical septal formation (D), corkscrew growth of the cell envelope in the growth region near the division site (E), and septal malformation and cell-wall formation away from the cell surface (F). (Scale bars: 200 nm, except B Top and Bottom and F, 500 nm.)