Fig. 2.

Translocation of kor mRNA into axons of primary DRG neurons. (A) Maps of the constructs including kor gene, MS2-GFP-nls, 5′K3′K, and 5′tk3′SV40. CMV, CMV promoter; Ex, exon; Int, intron; Luc, luciferase; MS2, single-stranded RNA phage capsid protein MS2; MS2-B, MS2-binding site; NSL, nuclear localization signal; SV40, SV40 promoter; Tk, thymidine kinase promoter. (B) Cotransfection with MS2-GFP-nls and 5′tk3′SV40 (the negative control). (C) Cotransfection with MS2-GFP-nls and 5′K3′K. Two separate fields are shown. Areas of inserts are enlarged by three times and are shown at the lower left corners. Bright-field image. GFP signal is shown in green, and τ- immunoreactivity is shown in red.