Fig. 4.

The role of kor mRNA 5′ UTR in axonal translation. (A) A panel of KOR-GFP translational cassettes, including the wild type (5′KGFP3′K), the 3′-deleted (5′KGFP), the 5′-deleted (3′KGFP), and the 5′- and 3′-deleted (negative control). (B) Transfection conducted in the side compartments for each construct. (Scale bars, 25 μm.) (C) A statistic analysis of quantification of GFP signals for each construct was obtained. (For comparing 3′KGFP with 5′KGFP3′K, ∗, P = 0.00034388; n = 7. For comparing negative control with 5′KGFP3′K, ∗, P = 0.000012207; n = 7.) GFP signal is in green.