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. 2006 Dec 13;103(52):19794–19799. doi: 10.1073/pnas.0609671104

Fig. 2.

Fig. 2.

Cytoplasmic Ca2+ mobilization is necessary for enhanced TLR4-dependent JNK activation and apoptosis. (A) Macrophages were incubated for 0.25–2 h with the indicated reagents, alone or in combination: thapsigargin (Tg; 0.5 μM), fucoidan (Fuc; 50 μg/ml), A23187 (2 μg/ml), tunicamycin (Tn; 2 μg/ml), and LPS (500 pg/ml). Cell lysates were immunoblotted for Thr-183/Thr-185-phospho-JNK (P-JNK) and total JNK. (B) Macrophages were preincubated for 10 min with vehicle control or 15 μM BAPTA. The cells then were treated with 50 μg/ml acetyl-LDL (Ac-LDL) or acetyl-LDL plus 58035 (FC loading) for 12 h. Lysates were immunoblotted for Thr-183/Thr-185-phospho-JNK, and total JNK. (C) Macrophages were preincubated for 10 min with vehicle control or 5 μM BAPTA-AM. The cells then were incubated for 24 h with 50 μg/ml acetyl-LDL alone or in combination with 58035, 0.5 μM thapsigargin, or thapsigargin plus 50 μg/ml fucoidan. The cells were assayed for apoptosis.