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. 2006 Dec 13;103(52):19658–19663. doi: 10.1073/pnas.0607245103

Fig. 5.

Fig. 5.

CD spectra and UV meltings of i-motif and 2-Ap-modified i-motif. (A) CD spectra of i-motif (1 μM in strand) not labeled (black line) or labeled with 2-Ap in the T(2-Ap)A loop positioned near the 5′ end in the major groove (open squares), in the narrow groove (open circles), and near the 3′ end in the major groove (open triangles) in aqueous cacodylic buffer (0.1 mM cacodylic acid/sodium cacodylate/100 mM NaCl, pH 5.5). (B) UV melting profiles of i-motif (9.2 μg/ml for i-motif and 9.5 μg/ml for G-quadruplex in base) not labeled (black line) or labeled with 2-Ap in the T(2-Ap)A loop positioned near the 5′ end in the major groove (open squares), in the narrow groove (open circles), and near the 3′ end in the major groove (open triangles) in aqueous cacodylic buffer (0.1 mM cacodylic acid/sodium cacodylate/100 mM NaCl, pH 5.5). DNA concentration was 9.2 μg/ml for i-motif and 9.5 μg/ml for G-quadruplex in base. Normalized absorption changes at 260 nm were plotted against temperature.