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. 2006 Dec 14;103(52):19812–19817. doi: 10.1073/pnas.0605768103

Fig. 2.

Fig. 2.

Wnt/β-catenin signaling negatively regulates cardiogenesis in the late stage of ES cell differentiation. (A) Western blot analysis for cTnT on day 10. EBs were cultured in DM or DM containing the indicated Wnt inhibitors from day 5. Wnt inhibitor treatment after day 5 increased cTnT expression. (B) MF20 and Topro staining on day 10. (Magnification: ×100.) (C) Morphometry for the expression of sarcomeric MHC in each EB. ∗, P < 0.01 vs. control. (D) RT-PCR analysis of cardiac, hematopoietic, and vascular marker genes. EBs were cultured in DM or DM containing Wnt inhibitors (DM + W.I.) or Wnt3a protein (DM + Wnt3a) from day 5. W.I., Wnt inhibitors. (E) Western blot analysis for cTnT on day 10. EBs were cultured in DM or DM containing Wnt3a (DM + Wnt3a) or BIO (DM + BIO) from day 5. Wnt3a treatment in the late stage of ES cell differentiation blocks further differentiation into mature cardiomyocytes. (F) MF20 and Topro staining on day 10. (Magnification: ×100.)